[Replication of a model of injury to human renal proximal tubular cells induced by hypoxia/reoxygenation].

Abstract

OBJECTIVE:

To replicate a new model of injury to human renal proximal tubular cells (HK-2) induced by hypoxia/reoxygenation.

METHODS:

Human renal proximal tubular cell line HK-2 cell was used as the target cell. Tubular cells were divided into six groups: 4 hours of hypoxia, 12 hours of hypoxia, 24 hours of hypoxia, and 24 hours of hypoxia followed by reoxygenation 4, 12 or 24 hours later groups. Each group was accompanied by a control group. Hypoxic culture conditions were produced by covering the culture with liquid paraffin. Trypan blue exclusion was used for cell count and cell viability. The activity of lactate dehydrogenase (LDH) in the culture medium was determined by biochemical method.

RESULTS:

After being challenged by hypoxia followed by reoxygenation, trypan blue exclusion rate was greater, cell count and cell viability were lower, and the activity of LDH was increased. It indicated that the destruction of integrity of cellular membrane was induced by ischemia/reperfusion injury, and the tubular cells may be injured irreversibly.

CONCLUSION:

A simple model of hypoxic injury of renal tubular cells is replicated by covering the culture cells with liquid paraffin.

• Authors: Yi-ting Du, Wen-bin Dong, Ming-yong Wang, Yong-lun Hang
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 10 Pg. 619-22 (Oct 2005) ISSN: 1003-0603 [Print] China
• PMID: 16259924 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• L-Lactate Dehydrogenase

Topics

• Cell Hypoxia
• Cell Line
• Cell Survival
• Humans
• Kidney Tubules, Proximal (cytology, enzymology)
• L-Lactate Dehydrogenase (metabolism)