Abstract | OBJECTIVE: METHODS:
Iodized oil with MCA and DEN was instilled into the left bronchi of 80 Wistar rats to induce squamous cell lung carcinoma. Iodized oil without MCA and DEN was instilled into the left bronchi of 10 rats as control group. Sixteen rats in the experimental group and 2 rats in the control group were killed 10, 15, 35, 60, and 270 days after experiment respectively to undergo pathological examination. Immunohistochemistry was used to detect the protein expression of COX-2 and caspase-3 in the bronchial endothelial cells. Immunohistochemical scores (IHS) were calculated. RESULTS: Pathological changes of different phases, such as hyperplasia of bronchial mucosal endothelial cells (14 cases), squamous metaplasia (25 cases), atypical proliferation (35 cases), carcinoma in situ (12 cases), infiltrative carcinoma (54 cases), and metastatic carcinoma (15 cases) appeared successively in the left lung mucosal endothelium of the experimental group. Weak expression of COX-2 protein was occasionally seen in the normal bronchial mucosal endothelium. COX-2 protein expression was becoming stronger and the IHS was becoming higher along with the development of carcinoma (P < 0.01). Caspase-2 protein expression was positive in 8 of the 10 (80%) of the control rats with an HIS of 5.92 +/- 0.9. And caspase-2 protein expression was becoming weaker along with the development of carcinoma (P < 0.01 or P < 0.05). Significant negative correlation was found between the COX-2 protein expression and caspase-3 expression (P < 0.01). CONCLUSION:
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Authors | Fu-chun Chen, Qi Pan, Yu-xia Zhang, Hong-lei Chen, Hong-gang Li, Hong-chao Tao, Fei Yang, Dong Xia, Ming-qiu Liu |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 85
Issue 27
Pg. 1916-20
(Jul 20 2005)
ISSN: 0376-2491 [Print] China |
PMID | 16255988
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclooxygenase 2
- Ptgs2 protein, rat
- Casp3 protein, rat
- Caspase 3
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Topics |
- Animals
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Caspase 3
(metabolism)
- Cyclooxygenase 2
(metabolism)
- Female
- Lung
(metabolism, pathology)
- Lung Neoplasms
(metabolism, pathology)
- Male
- Neoplasm Metastasis
- Rats
- Rats, Wistar
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