Wound infection after prosthetic material implantation is a troublesome complication with an incidence of 2% to 10%. The effect of
granulocyte colony-stimulating factor (
G-CSF) was studied in an experimental methicillin-resistant Staphylococcus aureus (MRSA) graft
infection model. Eighty adult mice were used. Under
general anesthesia an abdominal incision of 2 cm in length was performed. A subcutaneous cavity of 2 x 2 cm in size was created.
Polypropylene mesh pieces of 2 x 1 cm and MRSA
solution of 0.1ml of 10(8) CFU/mL were used.
G-CSF was applied systemically or locally in a dosage of 0.02 MU/30 g
body weight. There were 8 groups: group I,
wound + MRSA; group II,
wound + mesh + MRSA; group III,
wound + mesh + MRSA +
G-CSF (ip, 48 h before operation); group IV,
wound + mesh + MRSA +
G-CSF (
ip, 24 h before operation); group V,
wound + mesh + MRSA +
G-CSF (locally, into the cavity); group VI,
wound + mesh (incubated in
G-CSF solution for 4 h) + MRSA; group VII,
wound + mesh + MRSA +
G-CSF,
ip, 24 h from operation; and group VIII (positive control group),
wound + mesh + MRSA +
Teicoplanin (0.03 mg/30 g
body weight, ip, 1/2 h before operation). Three days after, animals were killed and incisions were examined for possible
infection or
abscess formation and
wound failure. Meshes were removed; after vortexing and dilution, samples were incubated with 5%
agar media. Results of bacterial incubation were evaluated 24 h and 48 h later. There were symptoms of
wound infection and
abscess formation in all groups except group VIII. In group VIII, MRSA was isolated in 7 events with a colony count below 10(3). Bacterial counts were above 10(6) (10(6)-10(8)) in all other groups. Thus, it was observed that
wound infection could be created with this model, but
G-CSF could not prevent the development of
wound infection, whether it was administered systemically or locally.
Teicoplanin decreased the number of colony-forming units of MRSA, and prevents
wound infection in this MRSA
wound infection model.