We aimed to investigate the protective effects of
trapidil after the occlusion of abdominal aorta and the
reperfusion injury in lung. Eighteen New Zealand albino rabbits were used in the study. In six animals [group 1,
ischemia-reperfusion (IR) group], the abdominal aorta was exposed and a microvascular clamp was placed in the infrarenal abdominal aorta for 60 min. After the ischemic period, the microvascular clamp was removed and reperfusion was provided for 2 h. After the reperfusion period, the lungs were removed carefully and specimens were prepared for histopathological and biochemical studies in appropriate conditions. In group 2 (study group),
trapidil (Rocarnal, Rentschler-UCB GmbH, Kerpen, Germany) was administered intraperitoneally as a single dose 1 h prior to trial, the IR procedure was performed and lung specimens were prepared similar to group 1. In group 3 (
sham group), the infrarenal abdominal aorta was exposed and lung specimens were prepared for histopathological and biochemical studies at the end of the study. Histopathological changes,
malondialdehyde (MDA),
nitric oxide (NO) and total sulfhydryl group (T-SH) levels were evaluated. There was a statistical difference between the IR group and study group regarding NO and MDA levels (P < 0.05 and P < 0.01, respectively), but this was not detected between the IR group and the
sham group (P > 0.05). There was no statistical difference among the three groups regarding T-SH levels (P > 0.05). While a statistical difference was found between the
sham group and study group in the NO level (P < 0.05), no statistical difference was found in the MDA level (P > 0.05). There was a statistical difference in interstitial
edema, PMN infiltration and
hemorrhage scores among the groups (P < 0.05). There was a statistical difference between the IR group and study group in PMN infiltration (P < 0.05), but this was not detected between the groups in interstitial
edema and
hemorrhage scores (P > 0.05). There was a statistical difference between IR group and
sham group in interstitial
edema, PMN infiltration and
hemorrhage scores (P < 0.05). Statistical difference was found between the
sham group and study group in interstitial
edema and
hemorrhage scores (P < 0.05), but not in PMN infiltration (P > 0.05).
CONCLUSIONS: