HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Oral administration of geranylgeranylacetone plus local somatothermal stimulation: a simple, effective, safe and operable preconditioning combination for conferring tolerance against ischemia-reperfusion injury in rat livers.

AbstractAIM:
To explore a simple, effective, safe and operable pretreatment for conferring tolerance against ischemia-reperfusion (I-R) injury in rat livers.
METHODS:
Forty-five rats were divided into five groups (each group n = 9). Group C: control group; group G: geranylgeranylacetone (GGA) was administered without heat stress; group S: local heat stress alone; group WG: GGA plus whole-body heat stress; group SG: GGA administration plus local heat stress. After completion of the I-R procedure, the ischemic-reperfused liver lobes in five groups were resected and tested for heat shock protein (HSP70) by RT-PCR, Western blotting analysis and immunohistochemical staining. The blood samples were collected for ALT and AST measurement at the end of occlusion of blood supply, 30 min after reperfusion, 24, 48, 72 h after surgery from the inferior vena cava. Survival was monitored for 1 wk.
RESULTS:
The production of HSP70 after I-R injury increased, the liver enzyme levels after reperfusion decreased rapidly, and the survival rates increased in groups C-SG.
CONCLUSION:
The combination of GGA plus local somatothermal stimulation is a simple, effective, safe and operable pretreatment to induce HSP70 in patients with liver tumor and cirrhosis before hepatectomy and in donors before harvesting graft for liver transplantation.
AuthorsNing Fan, Guang-Shun Yang, Jun-Hua Lu, Ning Yang, Hai-Bin Zhang
JournalWorld journal of gastroenterology (World J Gastroenterol) Vol. 11 Issue 36 Pg. 5725-31 (Sep 28 2005) ISSN: 1007-9327 [Print] United States
PMID16237775 (Publication Type: Journal Article)
Chemical References
  • Diterpenes
  • HSP70 Heat-Shock Proteins
  • RNA, Messenger
  • geranylgeranylacetone
Topics
  • Administration, Oral
  • Animals
  • Diterpenes (administration & dosage, pharmacology)
  • Gene Expression Regulation
  • HSP70 Heat-Shock Proteins (genetics, metabolism)
  • Heat-Shock Response
  • Hyperthermia, Induced
  • Ischemic Preconditioning (methods)
  • Liver (drug effects, metabolism)
  • Male
  • RNA, Messenger (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury (prevention & control)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: