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Serodiagnosis of neurocysticercosis using synthetic 8-kD proteins: comparison of assay formats.

Abstract
The assay of choice for serological detection of cysticercosis in humans and pigs is the enzyme-linked immunoelectrotransfer blot (EITB), a Western blot assay that relies on the use of seven lentil-lectin-purified glycoproteins (LLGPs) derived from Taenia solium metacestodes. The EITB is has a sensitivity of 98% and a specificity of 100% in detecting cysticercosis, yet scarcity of native source material and the labor-intensive process of metacestode purification hinder its practicality. These limitations have necessitated the reproduction of the EITB antigens in synthetic forms. Four chemically synthesized LLGP antigens, TS14, TS18var1, TSRS1, and TSRS2var1, were assayed individually by enzyme-linked immunosorbent assay (ELISA) and Western blot for immunoreactivity against a large cohort of sera from clinically defined neurocysticercosis patients. The sensitivity and specificity of all four of these antigens using the ELISA format were well below the standards set by the LLGP EITB, whereas results of the Western blot format closely mirrored those of the LLGP EITB.
AuthorsChristina M Scheel, Azra Khan, Kathy Hancock, Hector H Garcia, Armando E Gonzalez, Robert H Gilman, Victor C W Tsang, Cysticercosis Working Group in Peru
JournalThe American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 73 Issue 4 Pg. 771-6 (Oct 2005) ISSN: 0002-9637 [Print] United States
PMID16222024 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Helminth
  • Antigens, Helminth
  • Helminth Proteins
Topics
  • Amino Acid Sequence
  • Animals
  • Antibodies, Helminth (blood)
  • Antigens, Helminth (chemistry, immunology)
  • Blotting, Western (methods)
  • Enzyme-Linked Immunosorbent Assay
  • Helminth Proteins (chemical synthesis, chemistry, immunology)
  • Humans
  • Molecular Sequence Data
  • Neurocysticercosis (diagnosis)
  • Sensitivity and Specificity
  • Serologic Tests

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