Abstract | BACKGROUND: It has been hypothesized that in renal failure, exogenous glycation compounds from food accumulate and play a major pathogenetic role when renal excretion is impaired. METHODS: To address this, a diet containing a defined amount of the lysine Amadori product (AP) lactuloselysine was used. Plasma concentrations and cumulative urinary excretion of AP were assessed in 16 healthy subjects, 12 renal failure patients and 6 continuous ambulatory peitoneal dialysis ( CAPD) patients. Amadori product was measured as furosine using reverse phase high performance liquid chromatography (RP-HPLC) after acid hydrolysis. RESULTS: A diet low in glycation compounds significantly decreased excretion of APs in healthy subjects. In healthy individuals, ingestion of lactuloselysine bound to food proteins caused only a minor acute increase (8.24+/-1.11 mg/day, 2% of the administered dose) of AP excretion in the urine; in patients with renal failure not yet on dialysis, the increase in AP excretion in the urine was significantly less (4.0+/-0.51 mg/day) and the same was true in CAPD patients (0.21+/-0.09 mg/day). The plasma concentration of total APs, i.e. the sum of APs as free amino acids and residues bound to plasma proteins, did not change in any of the three groups, however. CONCLUSION:
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Authors | Vedat Schwenger, Christian Morath, Kathrin Schönfelder, Wolfgang Klein, Kai Weigel, Reinhold Deppisch, Thomas Henle, Eberhard Ritz, Martin Zeier |
Journal | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
(Nephrol Dial Transplant)
Vol. 21
Issue 2
Pg. 383-8
(Feb 2006)
ISSN: 0931-0509 [Print] England |
PMID | 16204288
(Publication Type: Journal Article)
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Chemical References |
- epsilon-N-1-(1-deoxylactulosyl)lysine
- Lactulose
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Topics |
- Administration, Oral
- Glycosylation
- Humans
- Lactulose
(administration & dosage, analogs & derivatives, blood, metabolism)
- Peritoneal Dialysis, Continuous Ambulatory
- Renal Insufficiency
(blood, complications, metabolism, therapy)
- Uremia
(blood, etiology, metabolism)
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