Biodetermination of N-(deacetyl-O-4-vinblastoyl-23)-L-tryptophan, a metabolite of vintriptol, by high-performance liquid chromatography with fluorescence detection.

Abstract

The determination of N-(deacetyl-O-4-vinblastoyl-23)-L-tryptophan (vintriptol acid, VtrpA), a metabolite of the investigational semi-synthetic vinca alkaloid vintriptol [N-(deacetyl-O-4-vinblastoyl-23)-L-ethyltryptophan, VtrpE], in plasma and urine samples is described. Sample pretreatment included liquid-liquid extraction of the buffered (pH 5.0) biological samples with chloroform-2-propanol (95:5, v/v). The analyses were performed by ion-exchange high-performance liquid chromatography on normal-phase silica with fluorescence detection. The assay was applied to the analysis of samples from cancer patients who had been treated with VtrpE in a phase I clinical study. VtrpA was found to be a principal metabolite of VtrpE with up to 1.2% of the administered dose excreted in the urine.

• Authors: O van Tellingen, J H Beijnen, R Baurain, W W ten Bokkel Huinink, H R van der Woude, W J Nooyen
• Journal: Journal of chromatography (J Chromatogr) Vol. 574 Issue 2 Pg. 293-8 (Feb 14 1992) Netherlands
• PMID: 1618962 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• vintriptol
• Vinblastine
• vintriptol acid

Topics

• Antineoplastic Agents (metabolism)
• Chromatography, High Pressure Liquid
• Humans
• Reproducibility of Results
• Spectrometry, Fluorescence
• Vinblastine (analogs & derivatives, blood, metabolism, urine)