Abstract | OBJECTIVE: To study the histochemical staining in the diagnosis of osteosarcoma. METHODS: RESULTS: With modified Ponceau trichrome staining, bone formation tissues showed a homogenous, orange-red interblended with blue in color. From osteoid to mature bone the color changed from orange-red, light blue to dark blue. Fibrotic tissue was stained blue in color with striated appearance. Cartilage was not stained. Picrosirius red method gave bone formation tissues homogenous staining. Along with bone maturation, from osteoid tissue to mineralized bones, the color showed changes from light red, yellow, orange-red, red to dark purple. The cartilage demonstrated homogenous light red in color. Fibrous tissue stained red interblended with yellow in color, striated in shape. With Masson trichrome staining osteoid displayed pale blue and mineralized bone showed dark blue in color. Fibrotic tissue showed a striated blue staining. CONCLUSION: The modified Ponceau trichrome and Picrosirius red staining methods are better than Masson trichrome to demonstrate bone formation tissue in osteosarcoma. The former two methods could be also used in study on bone formation.
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Authors | Qing Li, Xi-qi Gong, Fu-cheng Ma, Yi-ling Zhao, Xiao-hui Zhu |
Journal | Zhonghua zhong liu za zhi [Chinese journal of oncology]
(Zhonghua Zhong Liu Za Zhi)
Vol. 27
Issue 8
Pg. 489-91
(Aug 2005)
ISSN: 0253-3766 [Print] China |
PMID | 16188149
(Publication Type: English Abstract, Journal Article)
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Topics |
- Bone Neoplasms
(pathology)
- Histocytochemistry
- Humans
- Osteosarcoma
(pathology)
- Staining and Labeling
(methods)
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