[Correlation between the activation of AP-1 signal transduction pathway and metastasis of colorectal carcinoma].

Abstract

OBJECTIVE:

To investigate the binding activity of activator protein-1 (AP-1) with DNA probe in the colorectal carcinoma (CRC) tissues and surrounding tissues and explore the correlation between the activation of AP-1 signal transduction pathway and metastasis of CRC.

METHODS:

The AP-1 DNA binding activities were investigated by electrophoretic mobility shift assay (EMSA) in CRC specimens (T), surrounding tissues including 2 cm (P(2)), 5 cm(P(5)) far away from primary tumor margin and distal resection margin of the specimens (N). The mRNA expression level of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) were measured by quantitive reverse transcription polymerase chain reaction (Q- RT-PCR).

RESULTS:

The AP-1 DNA binding activity in T was significantly higher than those in P(2), P(5) and N (P< 0.05) tissues. There were significantly positive correlations between AP-1 DNA binding activity in tumor and invasive degree, lymphatic metastasis respectively (P< 0.01), but no correlation with histological classification and differentiation (P> 0.05). The transcription levels of VEGF and MMP-9 in CRC were significantly higher than those in P(5) and N (P< 0.01, P< 0.05) tissues. The transcription levels of VEGF and MMP-9 were significantly correlated with increasing AP-1 DNA binding activity (P< 0.01).

CONCLUSIONS:

AP-1 is significantly correlated to the invasion and metastasis in CRC. The activation of AP-1 signal transduction pathway might be involved in the angiogenesis and of degradation extracellular matrix during tumor metastasis.

• Authors: Wei-dong Li, Tian-shu Liu, Qing-juan Yao, Lie-chen Ji, Yan-ling Cui, Gang Liu, Li-wei Zhu, Peng-zhi Wang
• Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery (Zhonghua Wei Chang Wai Ke Za Zhi) Vol. 8 Issue 4 Pg. 356-9 (Jul 2005) ISSN: 1671-0274 [Print] China
• PMID: 16167262 (Publication Type: English Abstract, Journal Article)

Chemical References

• Transcription Factor AP-1
• Vascular Endothelial Growth Factor A
• Matrix Metalloproteinase 9

Topics

• Colorectal Neoplasms (metabolism, pathology)
• Female
• Humans
• Lymphatic Metastasis
• Male
• Matrix Metalloproteinase 9 (metabolism)
• Neoplasm Staging
• Neovascularization, Pathologic
• Signal Transduction
• Transcription Factor AP-1 (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)