Down-regulation of SMT3A gene expression in association with DNA synthesis induction after X-ray irradiation in nevoid basal cell carcinoma syndrome (NBCCS) cells.
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| Abstract |
Fibroblast cells derived from nevoid basal carcinoma syndrome (NBCCS) patients show increased levels of DNA synthesis after X-ray irradiation. Genes, whose expression is modulated in association with the DNA synthesis induction, were searched by using PCR-based mRNA differential display analysis in one of the NBCCS cell lines, NBCCS1 cells. Decreased levels of SMT3A gene expression were found in X-ray-irradiated NBCCS1 cells. This decrease was also shown by RT-PCR analysis in another cell line, NBCCS3 cells. In addition to NBCCS cells, normal fibroblast cells showed the DNA synthesis induction after X-ray irradiation when they were treated with antisense oligonucleotides (AO) for SMT3A. However, treatment of normal fibroblasts with the random oligonucleotides (RO) resulted in decreased levels of DNA synthesis after X-ray irradiation. Thus, down-regulation of SMT3A gene expression may be involved in the DNA synthesis induction after X-ray irradiation in the NBCCS cells at least tested.
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| Authors | Shigeru Sugaya, Hiroshi Nakanishi, Hideki Tanzawa, Katsuo Sugita, Kazuko Kita, Nobuo Suzuki |
| Journal | Mutation research (Mutat Res) Vol. 578 Issue 1-2 Pg. 327-32 (Oct 15 2005) ISSN: 0027-5107 [Print] Netherlands |
| PMID | 16154602 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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