Abstract | BACKGROUND/AIMS: Circulating anti- phospholipid antibodies (aPL) are often present in patients with alcoholic liver disease (ALD). The observations that defects in the disposal of apoptotic corpses leads to the development of aPL prompted us to investigate whether ALD-associated aPL might recognize antigens in apoptotic cells. METHODS: RESULTS: Flow cytometry revealed that IgG from ALD patients with high aPL titers selectively bind to the surface of apoptotic, but not to viable cells. No binding was instead evident using either control or aPL-negative ALD sera. ELISA assays using different oxidized phospholipids as antigens showed that anti- phospholipid reactivity of ALD sera was mainly directed towards oxidized cardiolipin and phosphatidylserine. The pre-adsorption of aPL-positive sera with oxidized phosphatidylserine, but not with oxidized cardiolipin, lowered aPL binding to apoptotic HuT-78 cells by about 50%. No effect was instead observed by pre-adsorption with oxidation-protected phospholipids or with human serum albumin adducted with different lipid peroxidation products. CONCLUSIONS: aPL associated with ALD target apoptotic cells by specifically recognizing oxidized phosphatidylserine, suggesting a possible link between hepatocyte apoptosis and anti- phospholipid auto-reactivity in ALD.
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Authors | Daria Vay, Cristina Rigamonti, Matteo Vidali, Elisa Mottaran, Elisa Alchera, Giuseppa Occhino, Massimo Sartori, Emanuele Albano |
Journal | Journal of hepatology
(J Hepatol)
Vol. 44
Issue 1
Pg. 183-9
(Jan 2006)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 16143424
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Antiphospholipid
- Phosphatidylserines
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Topics |
- Adult
- Aged
- Antibodies, Antiphospholipid
(immunology)
- Apoptosis
(physiology)
- Cell Membrane
(metabolism)
- Cells, Cultured
- Female
- Flow Cytometry
- Hepatocytes
(metabolism, ultrastructure)
- Humans
- Lipid Peroxidation
(physiology)
- Liver Diseases, Alcoholic
(immunology, metabolism, pathology)
- Male
- Middle Aged
- Oxidative Stress
(physiology)
- Phosphatidylserines
(metabolism)
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