Abstract | AIM: To study the effect of MHC class II transactivator(CIITA ) on the expression of MHC class I/II molecules, CD80 and CD86 molecules on dendritic cells(DCs), and explor the use of DCs modified by CIITA gene in the biotherapy of tumors. METHODS: We transfected the mouse CIITA gene by lipofectamine into DCs drived from mouse bone marrow. The expression of MHC class I/II, CD80, and CD86 molecules was detected by flow cytometry. 40 mice bearing hepatcellular carcinoma were divided into 4 groups: group 1 were treated with a para- tumor injection of PBS, group 2 DCs, group 3 modified DCs, and group 4 modified DCs pulsed with H22 tumor antigen. Then the anti- tumor effect of DCs modified by mCIITA was evaluated by measuring the tumor size. RESULTS: After transfection of mCIITA, the expression rate of MHC class I/II molecules of DCs increased from 74.2%/66.7% to 93.6%/91.4%, and that of CD80/CD86 increased from 52.3%/60.5% to 89.7%/91.5%. After immunization, the growth of H22 tumors in group 4 mice was significantly inhibited compared with that in other three groups (P<0.05 at 3, 4, 5, 6, and 7 weeks versus groups 1, 2; P<0.05 at 5, 6, 7 weeks versus group 3). CONCLUSION: The expression of MHC I/II, CD80 and CD86 on DCs was regulated markedly by transfection of mCIITA. mCIITA transfection also enhanced the anti- tumor effect of DCs. Our results provided a new method for biotherapy of tumors with DCs.
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Authors | Qi-shui Ou, Qi Lin, Bin Lan, Ci-ren Guo |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
(Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)
Vol. 21
Issue 5
Pg. 580-2, 586
(Sep 2005)
ISSN: 1007-8738 [Print] China |
PMID | 16143061
(Publication Type: Journal Article)
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Chemical References |
- B7-1 Antigen
- B7-2 Antigen
- HLA Antigens
- MHC class II transactivator protein
- Nuclear Proteins
- Trans-Activators
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Topics |
- Animals
- B7-1 Antigen
(metabolism)
- B7-2 Antigen
(metabolism)
- Carcinoma, Hepatocellular
(immunology)
- Cell Line, Tumor
- Dendritic Cells
(metabolism, physiology)
- Disease Models, Animal
- Female
- Flow Cytometry
- HLA Antigens
(metabolism)
- Liver Neoplasms
(immunology)
- Mice
- Mice, Inbred BALB C
- Nuclear Proteins
(genetics, physiology)
- Trans-Activators
(genetics, physiology)
- Transfection
(methods)
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