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Functional analysis of cross-reactive immunoglobulin E antibodies: peanut-specific immunoglobulin E sensitizes basophils to tree nut allergens.

AbstractBACKGROUND:
Peanut and tree nuts are a major cause of food-induced anaphylaxis with an appreciable mortality. Co-sensitization to peanuts and tree nuts is a common clinical observation and may be because of peanut-specific serum IgE antibodies that cross-react with tree nut allergens. It is, however, unclear whether these cross-reactive IgE antibodies are involved in effector-cell activation.
OBJECTIVE:
To determine if cross-reactivity of peanut-specific IgE antibodies with tree nuts can cause effector cell activation using an in vitro basophil activation assay.
METHODS:
Two peanut allergic subjects with positive specific IgE for peanut and tree nuts (as measured by CAP-FEIA) were studied. Basophil activation to peanut and tree nuts, as indicated by CD63 expression, was assessed by flow cytometry to confirm co-sensitization to peanut and tree nuts. Inhibition ELISA using sera from the subjects was performed to detect peanut-specific IgE antibodies that cross-reacted with tree nut proteins. To determine whether cross-reactive tree nut allergens can induce effector-cell activation, peanut-specific antibodies were affinity purified from the subject sera and used to resensitize non-peanut/tree nut allergic donor basophils stripped of surface IgE. Basophil activation was then measured following stimulation with peanut and tree nut extracts.
RESULTS:
The two peanut allergic subjects in this study showed positive basophil activation to the peanut and tree nut extracts. Inhibition ELISA demonstrated that pre-incubation of the peanut allergic subject sera with almond, Brazil nut and hazelnut extracts inhibited IgE binding to peanut extract. IgE-stripped basophils from non-peanut/tree nut allergic subjects resensitized with affinity-purified peanut-specific antibodies from the peanut allergic subject sera became activated following stimulation with peanut, almond and Brazil nut extracts, demonstrating biological activity of cross-reactive IgE antibodies.
CONCLUSION:
Peanut-specific IgE antibodies that cross-react with tree nut allergens can cause effector-cell activation and may contribute to the manifestation of tree nut allergy in peanut allergic subjects.
AuthorsM P de Leon, A C Drew, I N Glaspole, C Suphioglu, J M Rolland, R E O'Hehir
JournalClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 35 Issue 8 Pg. 1056-64 (Aug 2005) ISSN: 0954-7894 [Print] England
PMID16120088 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Allergens
  • Immunoglobulin E
Topics
  • Adolescent
  • Adult
  • Allergens (immunology)
  • Antibody Specificity (immunology)
  • Arachis (immunology)
  • Basophils (immunology)
  • Bertholletia (immunology)
  • Corylus (immunology)
  • Cross Reactions (immunology)
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Female
  • Humans
  • Immune Tolerance (immunology)
  • Immunity, Cellular (immunology)
  • Immunoglobulin E (immunology)
  • Middle Aged
  • Nuts (immunology)
  • Peanut Hypersensitivity (immunology)
  • Prunus (immunology)

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