Reversed-phase ion-paired HPLC of purine nucleotides from skeletal muscle, heart and brain of the goldfish, Carassius auratus L.--II. Influence of environmental anoxia on metabolite levels.

Abstract

1. The type and the amounts of some (di)nucleotides in white skeletal muscle, heart and brain of anoxic goldfish were established with a reversed-phase ion-paired HPLC method. 2. Significant changes in the levels of these metabolites, as compared to controls, were observed. The most consistent change is the increase of IMP and of IMP-load (IMP/ATP + ADP + AMP) in the three tissues during anoxia. 3. Adenylate energy charges are maintained at a high level in the anoxic white muscle and the anoxic heart. Comparison of these results with those from conventional enzymatic methods for the quantification of (di)nucleotides showed, except for IMP, no significant differences.

• Authors: J Van der Boon, R L de Jong, G E Van den Thillart, A D Addink
• Journal: Comparative biochemistry and physiology. B, Comparative biochemistry (Comp Biochem Physiol B) Vol. 101 Issue 4 Pg. 583-6 (Apr 1992) ISSN: 0305-0491 [Print] England
• PMID: 1611875 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Purine Nucleotides
• Oxygen

Topics

• Animals
• Brain (metabolism)
• Chromatography, High Pressure Liquid (methods)
• Goldfish
• Muscles (metabolism)
• Myocardium (metabolism)
• Oxygen (metabolism, physiology)
• Purine Nucleotides (metabolism)