[Apoptosis of pulmonary epithelial cells and endothelial cells in mice exposed to phosgene].

To study apoptosis of pulmonary epithelial cells and endothelial cells in mice with pulmonary edema induced by phosgene exposure.
Thirty-two mice were divided into normal group and phosgene group with 16 mice in each group. The mice in phosgene group were exposed to phosgene (11.9 mg/L) for 5 min and those in the control group to air. Four hours after exposure, alveolar type II cells were isolated and cultured to observe their apoptosis by electron microscope and flow cytometry. The lung tissues were also taken for DNA gel electrophoresis and TUNEL assay.
Apoptotic bodies were observed in alveolar type II cells under electron microscope in phosgene group, which had higher cell apoptosis rate than the control group [(40.26+/-7.74)% vs (1.58+/-1.01)%, P<0.001] as determined by flow cytometry. Ladder-like DNA fragmentation pattern was observed in DNA gel electrophoresis in phosgene group with apoptosis of the pulmonary epithelial and endothelial cells observed by TUNEL.
Phosgene can induce pulmonary epithelial and endothelial cell apoptosis in mice, suggesting that the mechanism of phosgene-induced pulmonary edema involves apoptosis of the lung cells.
AuthorsWen-li Li, Chun-xu Hai, Chen Yang, Bo Li, Rui Liu, Xiao-di Zhang
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA (Di Yi Jun Yi Da Xue Xue Bao) Vol. 25 Issue 8 Pg. 983-5, 990 (Aug 2005) ISSN: 1000-2588 [Print] China
PMID16109555 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chemical Warfare Agents
  • Phosgene
  • Animals
  • Apoptosis (drug effects)
  • Chemical Warfare Agents (toxicity)
  • Epithelial Cells (pathology)
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Phosgene (toxicity)
  • Pulmonary Alveoli (pathology)
  • Pulmonary Edema (chemically induced, pathology)
  • Random Allocation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: