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[Direct fluorescent and indirect immunofluorescent assay in buffy coat for candidemia diagnosis in pediatric patients: a comparative study].

Abstract
The diagnosis of candidemia by blood culture has poor sensitivity; therefore, immunossupresed patients and those with risk factors may receive empirical antifungal therapy, wich is potentially toxic. Fluorescent tests have been developed to obtain an early and more sensitive diagnosis than blood culture. The aim of this study was to compare indirect immunofluorescence vs direct calcofluor white fluorescence in buffy coat for candidemia diagnosis. Sensitivity, specificity, predictive values, of positive and negative samples were 60%, 86%, 33%, 95% and 90%, 80%, 35%, 99%, for indirect immunofluorescence and calcofluor white, respectively.
AuthorsEric Moisés Flores-Ruiz, María Guadalupe Miranda-Novales, Fortino Solórzano-Santos, Gustavo Sánchez-Huerta, Humberto Díaz-Ponce
JournalRevista iberoamericana de micología (Rev Iberoam Micol) Vol. 22 Issue 2 Pg. 102-4 (Jun 2005) ISSN: 1130-1406 [Print] Spain
Vernacular TitleFluorescencia directa e inmunofluorescencia indirecta en extensión leucocitaria para el diagnóstico de candidemia en pacientes pediátricos: estudio comparativo.
PMID16107168 (Publication Type: Clinical Trial, Comparative Study, Controlled Clinical Trial, English Abstract, Journal Article)
Chemical References
  • Benzenesulfonates
  • Fluorescent Dyes
  • C.I. Fluorescent Brightening Agent 28
Topics
  • Adolescent
  • Benzenesulfonates (analysis)
  • Candidiasis (blood, diagnosis)
  • Child
  • Child, Preschool
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Fluorescent Dyes (analysis)
  • Fungemia (blood, diagnosis)
  • Humans
  • Immunocompromised Host
  • Infant
  • Infant, Newborn
  • Leukocytes (microbiology)
  • Male
  • Microscopy, Fluorescence
  • Predictive Value of Tests
  • Sensitivity and Specificity

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