Platelet and granulocyte alloimmunisation in multitransfused Tunisian patients.

Abstract

We determined the frequency of post-transfusion alloimmunisation against platelet and granulocyte antigens in 51 Tunisian polytransfused patients with haematological diseases. Serum samples were analysed by a standard and an antiglobulin-augmented lymphocytotoxicity technique, a granulocyte agglutination test, a granulocyte immunofluorescence test, a platelet immunofluorescence test and the monoclonal antibody-specific immobilisation of platelet antigens assay. No granulocyte-specific antibodies were detected. HLA antibodies were found in 58.8% of patients. Platelet-specific antibodies were detected in four patients and were directed against human platelet antigen (HPA)-5b, HPA-1b and HPA-3a. The three patients with Glanzmann's thrombasthenia developed anti-GPIIb/IIIa antibodies. This study provides immunogenetic information that could improve the management of transfusion therapy in Tunisia.

• Authors: Hadef Skouri, Ali Bettaieb, Patricia Fromont, Halima Elomri, Souad Ennabli, Philippe Bierling
• Journal: European journal of haematology (Eur J Haematol) Vol. 75 Issue 3 Pg. 248-51 (Sep 2005) ISSN: 0902-4441 [Print] England
• PMID: 16104882 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright Blackwell Munksgaard 2005.

Chemical References

• Autoantibodies
• HLA Antigens

Topics

• Autoantibodies (blood)
• Blood Platelets (immunology)
• Granulocytes (immunology, transplantation)
• HLA Antigens (immunology)
• Humans
• Platelet Transfusion
• Tunisia