Study of fibrinogen adsorption on self-assembled monolayers on Au(111) by atomic force microscopy.

Abstract

The adsorption of plasma protein fibrinogen on the self-assembled monolayers (SAMs) of n-hexadecyl mercaptan and citrate was investigated with atomic force microscopy (AFM). On the basis of the preparation of these two flat SAMs on Au(111), high-resolution AFM images of bovine fibrinogen were obtained with different protein concentrations. The results indicated that the surface chemical composition significantly affected the adsorption behavior of fibrinogen. Since fibrinogen plays a key role in the regulation of both haemostasis and thrombosis, high-resolution AFM imaging on SAMs is expected to be an effective approach to study the haemocompatibility of materials with different surface chemistry.

• Authors: Yi Lin, Jun Wang, Li-Jun Wan, Xiao-Hong Fang
• Journal: Ultramicroscopy (Ultramicroscopy) Vol. 105 Issue 1-4 Pg. 129-36 (Nov 2005) ISSN: 0304-3991 [Print] Netherlands
• PMID: 16095837 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Silver
• Fibrinogen

Topics

• Adsorption
• Animals
• Cattle
• Fibrinogen (pharmacokinetics)
• Microscopy, Atomic Force
• Silver (chemistry)
• Surface Properties