Abstract |
Our recent studies have demonstrated that generation of ROS is associated with choline deficiency (CD)-induced apoptosis in CWSV-1 cells, an immortalized rat hepatocyte that becomes tumorigenic by stepwise culturing in decreasing levels of choline. In the present study, we investigated the effect of CD on loss of mitochondrial membrane potential ( MMP), using the JC-1 probe by FASCAN assay. Our data demonstrate that MMP in CD-cultured cells was decreased in a time- and dose-dependent manner and that significant disruption occurred at 24 h, relative to high choline (HC, 70 microM) cultured cells. In order to investigate further the relationship among the CD-induced ROS, MMP collapse, and apoptosis, we examined the effects of different inhibitors on ROS production, MMP disruption, and apoptosis in CD or HC-cultured CWSV-1 cells. These data indicate that the disruption of MMP is an upstream event in CD-induced apoptosis, and mitochondrial dysfunction plays a key role in mediating CD-induced apoptosis in CWSV-1 cells.
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Authors | Wei-Xing Guo, Quentin N Pye, Kelly S Williamson, Charles A Stewart, Kenneth L Hensley, Yashige Kotake, Robert A Floyd, Robert H Broyles |
Journal | Free radical biology & medicine
(Free Radic Biol Med)
Vol. 39
Issue 5
Pg. 641-50
(Sep 01 2005)
ISSN: 0891-5849 [Print] United States |
PMID | 16085182
(Publication Type: Journal Article)
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Chemical References |
- Benzyl Compounds
- Boc-D-FMK
- Enzyme Inhibitors
- Free Radicals
- Hydrocarbons, Fluorinated
- Reactive Oxygen Species
- Rotenone
- Cyclosporine
- Caspases
- Choline
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Topics |
- Animals
- Apoptosis
- Benzyl Compounds
(pharmacology)
- Blotting, Western
- Caspases
(metabolism)
- Cell Separation
- Cells, Cultured
- Choline
(metabolism)
- Cyclosporine
(pharmacology)
- DNA Fragmentation
- Electron Transport
- Enzyme Inhibitors
(pharmacology)
- Flow Cytometry
- Free Radicals
- Hepatocytes
(metabolism, pathology)
- Hydrocarbons, Fluorinated
(pharmacology)
- Liver Neoplasms
(metabolism)
- Membrane Potentials
- Mitochondria
(metabolism, pathology)
- Rats
- Reactive Oxygen Species
- Rotenone
(pharmacology)
- Time Factors
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