Abstract | BACKGROUND: METHODS:
Tumor sections from 79 patients who underwent endoscopic mucosal resection for early intestinal-type gastric carcinoma were stained using a COX-2-specific monoclonal antibody. Immunoblotting of COX-2 was used to assess the effects of bile acids on COX-2 expression and activity in human gastric cell lines. RESULTS: Among the 79 early gastric cancer lesions studied, 13 (16%) arose in the gastric pylorus. In this group, COX-2 immunoreactivity was negative to weak in 38% (5 of 13 lesions) and moderate to strong in 62% (8 of 13 lesions). In the control group, COX-2 immunoreactivity was negative to weak in 70% (46 of 66 lesions) and moderate to strong in 30% (20 of 66 lesions). COX-2 expression was significantly elevated in early gastric cancer located in the gastric pylorus, compared with that in the other locations. In human gastric cell lines, bile acids induced COX-2 expression, mediated by the ERK 1/2 mitogen-activated protein kinase pathway. CONCLUSIONS:
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Authors | Hiroshi Yasuda, Masaya Yamada, Yutaka Endo, Kazuaki Inoue, Makoto Yoshiba |
Journal | Journal of gastroenterology
(J Gastroenterol)
Vol. 40
Issue 7
Pg. 690-7
(Jul 2005)
ISSN: 0944-1174 [Print] Japan |
PMID | 16082585
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Membrane Proteins
- Cyclooxygenase 2
- PTGS2 protein, human
- Prostaglandin-Endoperoxide Synthases
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Topics |
- Adenocarcinoma
(genetics, pathology)
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(analysis)
- Biopsy, Needle
- Blotting, Western
- Cyclooxygenase 2
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Male
- Membrane Proteins
- Middle Aged
- Neoplasm Staging
- Probability
- Prognosis
- Prostaglandin-Endoperoxide Synthases
(genetics, metabolism)
- Pylorus
(pathology)
- Retrospective Studies
- Sampling Studies
- Sensitivity and Specificity
- Statistics, Nonparametric
- Stomach Neoplasms
(genetics, pathology)
- Tumor Cells, Cultured
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