Evaluation of reduced toxicity of zearalenone by extrusion processing as measured by the MTT cell proliferation assay.
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| Abstract |
The objective of this study was to determine loss of toxicity of zearalenone in extruded cereal-based products by the MTT (tetrazolium salt) cell proliferation assay using a sensitive MCF-7 human breast cancer cell line and to compare the results to chemical (high-performance liquid chromatography, HPLC) and biochemical (enzyme-linked immunosorbent assay, ELISA) methods of analysis. A split-split plot design was used for the extrusion process experiments at temperatures of 150, 175, and 200 degrees C and screw speeds of 70 and 140 rpm. The initial zearalenone concentration in the artificially contaminated corn grits with Fusarium graminearum was found at a mean concentration of 37.88 microg/g as measured by HPLC. The percent reductions of zearalenone in the contaminated corn grits upon extrusion processing were in the ranges of 67-81, 60-72, and 66-78% as measured by HPLC, ELISA, and the MTT cell proliferation assay, respectively. The MTT cell proliferation assay results were more closely correlated with HPLC results (r = 0.96) than ELISA results (r = 0.83). The MTT cell proliferation assay was demonstrated to be a useful method for quantification of zearalenone as well as a potential toxicity screening method for contaminated extruded cereal-based products.
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| Authors | Yuksel Cetin, Lloyd B Bullerman |
| Journal | Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 53 Issue 16 Pg. 6558-63 (Aug 10 2005) ISSN: 0021-8561 [Print] United States |
| PMID | 16076149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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