Regular expression of discoidin domain receptor 2 in the improved adjuvant-induced animal model for rheumatoid arthritis.

Abstract

OBJECTIVE:

To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in improved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2's antagonist use clinically.

METHODS:

AIA was modified by administrating 0.1 mL of complete Freund's adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats' right hind paws and 0.125 mL tumor necrosis factor-alpha (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay.

RESULTS:

Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization.

CONCLUSION:

Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.

• Authors: Wei Li, Yuan-qiang Zhang, Xin-ping Liu, Li-bo Yao, Lan Sun
• Journal: Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih (Chin Med Sci J) Vol. 20 Issue 2 Pg. 133-7 (Jun 2005) ISSN: 1001-9294 [Print] China
• PMID: 16075756 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• BCG Vaccine
• Collagen Type II
• Receptors, Mitogen
• Freund's Adjuvant
• Discoidin Domain Receptors
• Receptor Protein-Tyrosine Kinases

Topics

• Animals
• Antibodies (blood)
• Arthritis, Experimental (etiology, metabolism)
• Arthritis, Rheumatoid (chemically induced, metabolism)
• BCG Vaccine
• Collagen Type II (immunology)
• Discoidin Domain Receptors
• Female
• Fibroblasts (drug effects, metabolism, pathology)
• Freund's Adjuvant
• Rats
• Rats, Sprague-Dawley
• Receptor Protein-Tyrosine Kinases (metabolism)
• Receptors, Mitogen (metabolism)
• Synovial Fluid (cytology, metabolism)