Quantitative immunodetection of key elements of polyphosphoinositide signal transduction in osteoblasts from arthritic patients shows a direct correlation with cell proliferation.

Abstract	Phosphoinositides play an essential role in diverse cellular functions such as cell proliferation, cytoskeletal regulation, intracellular vesicle trafficking, motility, cell metabolism and death. Alteration of these pathways is common to many diseases. In this study, we show that osteoblasts from patients affected by osteoarthritis (OA) and by rheumatoid arthritis (RA) present a decreased cell proliferation and a reduced expression of the key elements of polyphosphoinositide signal transduction such as phosphatidylinositol-3-kinase (PI 3K), phospholipase C gamma1 (PLCgamma1), and protein kinase C zeta (PKCzeta) compared to the post-traumatic (PT) patients. Our results suggest that a correlation may exist between the reduced osteoblast proliferation observed in OA and RA patients and the lowered expression of PI 3K, PLCgamma1, and PKCzeta enzymes. The reduced proliferation rate of osteoblasts in response to these signal transduction effectors could counteract the evolution of arthritic disease.
Authors	Nicoletta Zini, Gina Lisignoli, Liliana Solimando, Alberto Bavelloni, Aurelio Valmori, Sandra Cristino, Alberto Maria Martelli, Andrea Facchini, Nadir Mario Maraldi
Journal	Histochemistry and cell biology (Histochem Cell Biol) Vol. 124 Issue 2 Pg. 131-7 (Aug 2005) ISSN: 0948-6143 [Print] Germany
PMID	16052323 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Biomarkers Phosphatidylinositol Phosphates Phosphatidylinositol 3-Kinases protein kinase C zeta Protein Kinase C Type C Phospholipases Phospholipase C gamma
Topics	Aged Arthritis, Rheumatoid (enzymology, pathology) Biomarkers (metabolism) Cell Proliferation Cells, Cultured Female Femur Head (cytology) Fluorescent Antibody Technique, Indirect Humans Immunoenzyme Techniques Male Middle Aged Osteoarthritis (enzymology, pathology) Osteoblasts (metabolism, pathology) Phosphatidylinositol 3-Kinases (metabolism) Phosphatidylinositol Phosphates (metabolism) Phospholipase C gamma Protein Kinase C (metabolism) Signal Transduction Type C Phospholipases (metabolism) Wounds and Injuries (metabolism, pathology)

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