[The mechanism of the recipient maternal-fetal immuno-tolerance induced by the transferred paternal antigen-tolerant T cells].

Abstract

To study the effect of adoptive transfer of paternal antigen-tolerant T cells on recipient reactive T cells, CBA/JxDBA/2 mating was recruited as an abortion-prone model, and CBA/JxBALB/c mating as a successful pregnancy model. The abortion-prone CBA/J females mated with DBA/2 males were injected intraperitoneally with rat anti-mouse CD80 and CD86 mAb or rat isotype IgG at day 4 after gestation (time of implantation). The purified T cells were obtained from spleen of the pregnant CBA/J mice using magnetic beads at day 9 after gestation and labeled with CFSE in vitro. The CFSE-labeled T cells were intravenously injected into other CBA/J females mated with DBA/2 males at day 4 after gestation. The proliferation of recipient splenocytes in response to DBA/2 stimulator cells was evaluated at day 9 after gestation in vitro, and the expressions of intracellular cytokines and costimulatory molecules in CFSE +/- T cells were analyzed by flow cytometry. The results showed that adoptive transfer of either paternal antigen-tolerant T cells or T cells from BALB/c-mated CBA/J mice significantly suppressed the proliferation of recipient splenocytes in response to DBA/2 stimulator cells and resulted in lower frequency of cells positive for IL-2, IFN-gamma, CD28 and higher frequency of IL-10,CTLA-4-producing cells in both CFSE+ CD3+ population and CFSE- CD3+ population compared with adoptive transfer of T cells from isotype IgG-treated CBA/J mice, whereas the frequency of IL-4-producing cells did not appear significant change. Our findings suggest that paternal antigen-tolerant T cells transferred in recipient not only function as antigen-specific suppresser cells but also disable the recipient reactive T cells, which co-suppresses maternal rejection to the allogeneic fetus, thus resulting in the decrease of the embryo resorption rate of the abortion-prone mice to that of the normal pregnancy mice.

• Authors: Li Ping Jin, Da Jin Li, Ming Yan Wang, Xiao Yong Zhu, Ying Zhu, Yi Ming, Min Min Yuan
• Journal: Shi yan sheng wu xue bao (Shi Yan Sheng Wu Xue Bao) Vol. 38 Issue 3 Pg. 199-204 (Jun 2005) ISSN: 0001-5334 [Print] China
• PMID: 16044912 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• B7-1 Antigen
• B7-2 Antigen

Topics

• Adaptive Immunity (immunology)
• Animals
• Antibodies, Monoclonal (immunology)
• B7-1 Antigen (immunology)
• B7-2 Antigen (immunology)
• Female
• Immune Tolerance (immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Inbred CBA
• Mice, Inbred DBA
• Pregnancy
• Rats
• Spleen (immunology)
• T-Lymphocytes (immunology)