Abstract | BACKGROUND: Habitual arecanut chewing is associated with Oral Submucous Fibrosis (OSF). High copper content in arecanut plays a vital role in pathogenesis of OSF. This study evaluates the copper-staining pattern of buccal epithelial cells in oral cytological smears of non-chewers, chewers, and OSF. MATERIALS & METHODS: Alcohol fixed buccal epithelial smears of 10 histopathologically confirmed cases of OSF, 10 chewers without lesion, and 5 nonchewers were stained with modified Rhodamine technique and studied under the light microscope. Buccal epithelial smears of non-chewers dipped in copper sulphate solution were used as known positive for copper. RESULT:
Copper appeared as shades of palered within the cytoplasm of chewers and did not show any stain in non-chewers. Intense red stain was seen in OSF smears as dark granules within the cytoplasm. CONCLUSION: Intense staining of copper in OSF buccal smears, than in the chewers supports the role of copper in the pathogenesis of OSF.
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Authors | T Rooban, T R Saraswathi, Antony George, Elizabeth Joshua, K Ranganathan |
Journal | Indian journal of dental research : official publication of Indian Society for Dental Research
(Indian J Dent Res)
2004 Oct-Dec
Vol. 15
Issue 4
Pg. 129-32
ISSN: 0970-9290 [Print] India |
PMID | 16035640
(Publication Type: Journal Article)
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Chemical References |
- Fluorescent Dyes
- Rhodamines
- Copper
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Topics |
- Areca
- Copper
(analysis)
- Cytoplasm
(pathology)
- Epithelial Cells
(pathology)
- Fluorescent Dyes
- Humans
- Mouth Mucosa
(pathology)
- Oral Submucous Fibrosis
(pathology)
- Rhodamines
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