Matrilysin over-expression in MCF-7 cells enhances cellular invasiveness and pro-gelatinase activation.

Abstract	Matrilysin (MMP-7) is over-expressed in various cancers and is thought to play important roles in tumor invasion and metastasis. However, the function of MMP-7 in breast cancer remains unclear. We therefore examined the expression of the MMP-7 gene in breast cancer (MCF-7) cells and the effect of its over-expression on cellular invasion. We transfected human MMP-7 into MCF-7 cells and selected clones that stably over-expressed the MMP-7 gene. The in vitro invasiveness of MCF-7 cells was quantified by use of the Matrigel invasion assay. Expression of MMP-7 mRNA was analyzed by quantitative RT-PCR. MMP secretion and activation were detected by gelatin zymography. We found that MMP-7-expressing clones had significantly increased invasion (P < 0.001), with increased MMP-7 expression and gelatinase activation as compared to the vector controls. We conclude that MMP-7 over-expression correlates with breast cancer in vitro invasiveness and that MMP-7 may promote invasion by increasing the secretion and activation of proMMP-2 and proMMP-9.
Authors	Fengqiang Wang, Scott Reierstad, David A Fishman
Journal	Cancer letters (Cancer Lett) Vol. 236 Issue 2 Pg. 292-301 (May 18 2006) ISSN: 0304-3835 [Print] Ireland
PMID	16019136 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References	Drug Combinations Enzyme Precursors Laminin Proteoglycans RNA, Messenger matrigel Collagen Gelatinases Metalloendopeptidases progelatinase MMP7 protein, human Matrix Metalloproteinase 7 Matrix Metalloproteinase 2 Matrix Metalloproteinase 9
Topics	Breast Neoplasms (enzymology, genetics, pathology) Cell Line, Tumor Cell Movement (genetics) Collagen Drug Combinations Enzyme Activation Enzyme Precursors (metabolism) Gelatinases (metabolism) Humans Laminin Matrix Metalloproteinase 2 (metabolism) Matrix Metalloproteinase 7 (genetics, metabolism) Matrix Metalloproteinase 9 (metabolism) Metalloendopeptidases (metabolism) Neoplasm Invasiveness Proteoglycans RNA, Messenger (metabolism) Transfection

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