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Characterization of prolidase activity in erythrocytes from a patient with prolidase deficiency: comparison with prolidase I and II purified from normal human erythrocytes.

AbstractOBJECTIVES:
The purpose of this study was to investigate the effect of various amino acids and their metabolites on the activities of prolidase I and II from human erythrocytes compared to those in a patient with prolidase deficiency.
DESIGN AND METHODS:
Prolidase I and II from human erythrocytes were purified by using column chromatography. Prolidase activity against various iminodipeptides was determined by spectrophotometry using Chinard's method.
RESULTS:
The activities of prolidase I and II against glycylproline and methionylproline were enhanced by glycine, L- and D-isoforms of alanine and serine and D-isoforms of valine, leucine and isoleucine. L-isoforms of branched amino acids inhibited the activity of prolidase I. On the other hand, the activity of prolidase II was enhanced by all of these L-branched amino acids. The patient's prolidase activity was also enhanced by all the L- and D-branched amino acids.
CONCLUSION:
The activities of prolidase I and II against various iminodipeptides were prominently enhanced by glycine, but the effect of L-valine differed between the two enzymes. Enzymatic properties of the patient's prolidase were essentially the same as those of prolidase II.
AuthorsGang Liu, Kazuko Nakayama, Yasuhiro Sagara, Shiro Awata, Koichi Yamashita, Masanobu Manabe, Hiroyuki Kodama
JournalClinical biochemistry (Clin Biochem) Vol. 38 Issue 7 Pg. 625-31 (Jul 2005) ISSN: 0009-9120 [Print] United States
PMID16009141 (Publication Type: Journal Article)
Chemical References
  • Amino Acids
  • Chlorides
  • Dipeptides
  • Manganese Compounds
  • glycylproline
  • Dipeptidases
  • proline dipeptidase
  • Valine
  • manganese chloride
Topics
  • Amino Acids (pharmacology)
  • Chlorides (pharmacology)
  • Dipeptidases (blood, deficiency, isolation & purification)
  • Dipeptides (metabolism)
  • Electrophoresis, Polyacrylamide Gel
  • Erythrocytes (enzymology)
  • Humans
  • Kinetics
  • Manganese Compounds (pharmacology)
  • Stereoisomerism
  • Valine (pharmacology)

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