Abstract |
A genetically engineered strain of Pseudomonas putida U designed for the identification of new therapeutic herbicides has been obtained. In this bacterium, deletion of the homogentisate gene cluster (hmgRABC) confers upon this mutant huge biotechnological possibilities since it can be used: (i) as a target for testing new specific herbicides (p-hydroxy- phenylpyruvate dioxygenase inhibitors); (ii) to identify new therapeutic drugs-effective in the treatment of alkaptonuria and other related tyrosinemia - and (iii) as a source of homogentisic acid in a plant-bacterium association.
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Authors | Elsa Arias-Barrau, Angel Sandoval, Mario Arcos, Sagrario Arias, Germán Naharro, Elías R Olivera, José M Luengo |
Journal | FEMS microbiology letters
(FEMS Microbiol Lett)
Vol. 249
Issue 2
Pg. 297-302
(Aug 15 2005)
ISSN: 0378-1097 [Print] England |
PMID | 16006061
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Amino Acid Sequence
- Base Sequence
- Biotechnology
(methods)
- Chromatography, High Pressure Liquid
- DNA Primers
- Genetic Engineering
(methods)
- Herbicides
(pharmacology)
- Molecular Sequence Data
- Multigene Family
- Mutagenesis
- Pseudomonas putida
(drug effects, genetics)
- Restriction Mapping
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