Abstract | BACKGROUND:
Tumor necrosis factor-related, apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in a wide variety of tumor cells, but it does not cause toxicity in the majority of normal cells. Therefore, TRAIL could become a suitable agent for anticancer therapies. However, a number of tumor cell lines are known to be resistant to TRAIL-induced apoptosis. The purpose of this study was to determine the mechanisms of resistance to TRAIL in pancreatic cancer cells. METHODS: RESULTS:
Pancreatic cancer cells responded to TRAIL in a different way. Resistant cell lines, AsPC-1, Suit-2, and CFPAC-1, expressed higher levels of FLIP-S protein, one of the splice variants of FLIP. Cycloheximide reduced the expression of FLIP in the resistant cells. Combined treatment with cycloheximide and TRAIL induced cleaved forms of caspases and simultaneously restored the sensitivity to TRAIL-induced apoptosis in the resistant cells. CONCLUSIONS:
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Authors | Tomohiko Mori, Ryuichiro Doi, Eiji Toyoda, Masayuki Koizumi, Daisuke Ito, Kazuhiro Kami, Atsushi Kida, Toshihiko Masui, Yoshiya Kawaguchi, Koji Fujimoto |
Journal | Surgery
(Surgery)
Vol. 138
Issue 1
Pg. 71-7
(Jul 2005)
ISSN: 0039-6060 [Print] United States |
PMID | 16003319
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoptosis Regulatory Proteins
- Membrane Glycoproteins
- Protein Synthesis Inhibitors
- Receptors, TNF-Related Apoptosis-Inducing Ligand
- Receptors, Tumor Necrosis Factor
- TNF-Related Apoptosis-Inducing Ligand
- TNFRSF10A protein, human
- TNFRSF10B protein, human
- TNFSF10 protein, human
- Tumor Necrosis Factor-alpha
- Cycloheximide
- Caspases
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Topics |
- Apoptosis
(drug effects)
- Apoptosis Regulatory Proteins
- Caspases
(metabolism)
- Cell Line, Tumor
- Cycloheximide
(pharmacology)
- Drug Resistance, Neoplasm
- Gene Expression
(drug effects)
- Humans
- Membrane Glycoproteins
(pharmacology)
- Pancreatic Neoplasms
(drug therapy, pathology)
- Protein Synthesis Inhibitors
(pharmacology)
- Receptors, TNF-Related Apoptosis-Inducing Ligand
- Receptors, Tumor Necrosis Factor
(genetics)
- Signal Transduction
(drug effects)
- TNF-Related Apoptosis-Inducing Ligand
- Tumor Necrosis Factor-alpha
(pharmacology)
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