The aim of this study was to assess the inhibitory effect of whole
bee venom (BV) on adjuvant-induced
arthritis in the rat. Rats were divided into pre-
apitherapy, post-
apitherapy and control experimental groups. The pre-
apitherapy group was subcutaneously stung with a honeybee (Apis mellifera L.) and the control group was subcutaneously injected with 0.1 ml of physiological
saline solution one day prior to complete
Freund's adjuvant (CFA) injection. The post-
apitherapy group was subcutaneously stung with a honeybee on day 14 after CFA injection. When
arthritis had developed in the rat, the post-
apitherapy group was subcutaneously administered whole BV every other day for a further 14 days. Clinical signs, hematological values and radioglogical features were observed during the entire experimental period. In the pre-
apitherapy group, the development of inflammatory
edema and
polyarthritis was inhibited. Significant differences in lameness score, hind paw
edema volume and radiological features were observed between control and pre-
apitherapy rats. White blood cell counts indicated that the degree of leucocytosis was significantly different between the pre-
apitherapy and control groups (p < 0.01). Inflammatory
edema,
polyarthritis and bone change into the right hind paw were effectively inhibited in pre-
apitherapy rats during the two-week period post-CFA injection. In conclusion, whole BV was found to inhibit arthritic
inflammation and bone changes in the rat. This may be an alternative treatment for
arthritis in humans.