Abstract | PURPOSE: METHODS: Male and female marmosets (Callithrix jacchus) (n = 18), ages between 2 and 8 years, were injected with domoic acid (0.5-4 mg/kg, i.p.) or saline, and behaviorally assessed with regard to the presence of acutely induced seizures and for < or = 6 months for spontaneous seizures. Injection of doses ranging from 3.5 to 4 mg/kg either did not induce SE or resulted in fatal SE. Even a 5-min SE duration (SE blockade resulting from diazepam injection) proved lethal to marmosets within 1 h of domoate administration, regardless of intensive care and monitoring of the animals. Animals injected with doses ranging from 0.5 to 3 mg/kg that developed only a few minor convulsive signs were allowed a 6-month survival period for the assessment of spontaneous epileptic events. At the end of the experiment, 6-month period, or acute intoxication associated with SE induction, animals were deeply anesthetized and had their brains subjected to histologic processing for Nissl and delta-FosB. RESULTS: For the animals injected with domoate that did not develop SE (i.e., those that survived), we could not detect any behavioral signs of spontaneous epileptic seizures in the 6-month observation period, and only minor indications of neuropathologic changes (i.e., neuronal death) over Nissl-stained sections, as well as some small changes in the staining for delta-FosB in a few of the animals. CONCLUSIONS:
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Authors | Patricia Perez-Mendes, Simone M Cinini, Magda A Medeiros, Sergio Tufik, Luiz E Mello |
Journal | Epilepsia
(Epilepsia)
Vol. 46 Suppl 5
Pg. 148-51
( 2005)
ISSN: 0013-9580 [Print] United States |
PMID | 15987270
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Behavior, Animal
(drug effects, physiology)
- Brain
(drug effects, pathology, physiopathology)
- Callithrix
(physiology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Epilepsy, Temporal Lobe
(chemically induced, pathology, physiopathology)
- Female
- Follow-Up Studies
- Injections, Intraperitoneal
- Kainic Acid
(administration & dosage, analogs & derivatives, pharmacology, toxicity)
- Male
- Motor Activity
(drug effects, physiology)
- Neurotoxicity Syndromes
(etiology, mortality, pathology)
- Status Epilepticus
(chemically induced, pathology, physiopathology)
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