Consistent lack of CD34-positive stromal cells in the stroma of malignant breast lesions.

Abstract

To examine the distribution of CD34-positive and ASMA-positive stromal cells in various breast lesions, we performed immunohistochemical assays (using a streptavidin-biotin immunoperoxidase technique) of tissue specimens, obtained by excisional biopsy and partial or total mastectomy, from 62 patients with breast lesions. Specimens were obtained from 64 lesions as follows: fibrocystic disease (n=12), intraductal papilloma (n=4), fibroadenoma (n=17), invasive lobular carcinoma (n=6), invasive ductal carcinoma (n=20) and invasive micropapillary carcinoma (n=5). In normal breast tissue (controls), CD34-positive spindle cells were abundant in the intralobular stroma, but no ASMA-positive stromal cells were identified except myoepithelial cells. Small to large numbers of CD34-positive cells were observed in the stroma of 29 of 33 benign diseases. In all invasive carcinomas (lobular, ductal and micropapillary), no CD34-positive stromal cells were observed in the stroma. In the stroma of benign lesions, the number of ASMA-positive stromal cells was various, but the stroma of all invasive breast cancers contained ASMA-positive stromal cells. The present results indicate that disappearance of CD34-positive stromal cells consistently occurs in the stroma of invasive carcinoma of the breast, irrespective of histological type and may be associated with the presence of ASMA-positive stromal cells.

• Authors: N Kuroda, Y-L Jin, T Hamauzu, M Toi, E Miyazaki, M Hiroi, T Moriki, H Enzan
• Journal: Histology and histopathology (Histol Histopathol) Vol. 20 Issue 3 Pg. 707-12 (07 2005) ISSN: 0213-3911 [Print] Spain
• PMID: 15944918 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD34

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Antigens, CD34 (analysis)
• Breast (chemistry, pathology)
• Breast Neoplasms (metabolism, pathology)
• Carcinoma, Ductal, Breast (metabolism, pathology)
• Carcinoma, Intraductal, Noninfiltrating (metabolism, pathology)
• Female
• Fibroadenoma (metabolism, pathology)
• Fibrocystic Breast Disease (metabolism, pathology)
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Stromal Cells (chemistry, pathology)