Abstract |
The metabolism of arachidonic acid by either the cyclooxygenase or lipoxygenase (LOX) pathway is believed to play an important role in tumor promotion. We investigated the expression of 5- and 12-LOX in renal cell carcinoma (RCC), as well as the effects of their inhibitors on cell proliferation in 2 RCC cell lines (Caki-1 and A498). Expression of 5- and 12-LOX was detected by immunohistochemistry and RT-PCR. Effects of LOX inhibitors on RCC cell growth were examined by MTT assay, and Hoechst staining was used to determine whether or not the LOX inhibitors induce apoptosis. While 5- and 12-LOX expression levels were slightly detected in NK tissues, marked expressions of 5- and 12-LOX were detected in RCC tissues. 5-LOX inhibitors caused marked reduction of RCC cells in a concentration- and time-dependent manner. The effect of the 5-LOX inhibitor was stronger than the 12-LOX inhibitor. Furthermore, the 5-LOX inhibitor caused a marked reduction of RCC cells through apoptosis. LOX, especially 5-LOX, is induced in RCC, and the results suggest that the 5-LOX inhibitor may mediate potent anti-proliferative effects against RCC cells. Thus, 5-LOX may become a new target in treatment of RCC.
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Authors | Masahide Matsuyama, Rikio Yoshimura, Makoto Mitsuhashi, Kenji Tsuchida, Yoshiaki Takemoto, Yutaka Kawahito, Hajime Sano, Tatsuya Nakatani |
Journal | Oncology reports
(Oncol Rep)
Vol. 14
Issue 1
Pg. 73-9
(Jul 2005)
ISSN: 1021-335X [Print] Greece |
PMID | 15944770
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Flavonoids
- Lipoxygenase Inhibitors
- RNA, Messenger
- Arachidonic Acid
- baicalin
- Masoprocol
- Arachidonate 12-Lipoxygenase
- Arachidonate 5-Lipoxygenase
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Topics |
- Aged
- Apoptosis
(drug effects)
- Arachidonate 12-Lipoxygenase
(genetics, metabolism)
- Arachidonate 5-Lipoxygenase
(genetics, metabolism)
- Arachidonic Acid
(metabolism)
- Carcinoma, Renal Cell
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Cells, Cultured
- Dose-Response Relationship, Drug
- Female
- Flavonoids
(pharmacology)
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Kidney
(cytology, metabolism)
- Kidney Neoplasms
(genetics, metabolism, pathology)
- Lipoxygenase Inhibitors
(pharmacology)
- Male
- Masoprocol
(pharmacology)
- Middle Aged
- RNA, Messenger
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
- Time Factors
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