CpG motif in ATCGAT hexamer improves DNA-vaccine efficiency against lethal Pseudorabies virus infection in pigs.

Abstract

This study evaluated the adjuvant activity of a porcine-specific type A CpG-containing oligodeoxynucleotide (CpG-ODN) in a DNA-vaccine against Pseudorabies virus (PrV) infection. This vaccine, composed of three independent plasmids encoding PrV gB, gC and gD glycoproteins, was injected alone or was co-injected with CpG-ODN or control GpC-ODN. CpG-ODN improved the PrV-specific humoral immune response and provided better clinical protection against lethal PrV-infection. The PrV-specific cell-mediated immune response seemed also to be increased.

• Authors: Daniel Dory, Véronique Béven, Anne-Marie Torché, Stéphanie Bougeard, Roland Cariolet, André Jestin
• Journal: Vaccine (Vaccine) Vol. 23 Issue 36 Pg. 4532-40 (Aug 22 2005) ISSN: 0264-410X [Print] Netherlands
• PMID: 15921828 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Viral
• CPG-oligonucleotide
• Oligodeoxyribonucleotides
• Pseudorabies Vaccines
• RNA, Messenger
• Vaccines, DNA
• Interleukin-4
• Interferon-gamma

Topics

• Adjuvants, Immunologic (pharmacology)
• Animals
• Antibodies, Viral (blood)
• Body Weight
• Immunization
• Interferon-gamma (genetics)
• Interleukin-4 (genetics)
• Oligodeoxyribonucleotides (pharmacology)
• Pseudorabies (prevention & control)
• Pseudorabies Vaccines (immunology)
• RNA, Messenger (analysis)
• Swine
• Swine Diseases (prevention & control)
• Vaccines, DNA (immunology)