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[Feasibility of muscle-derived cell autotransplantation as a treatment for post-prostatectomy urinary incontinence].

AbstractOBJECTIVE:
To explore the feasibility of muscle-derived cell autotransplantation in the treatment of post-prostatectomy urinary incontinence.
METHODS:
Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining.
RESULTS:
At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells.
CONCLUSION:
Autotransplanted MDCs can survive permanently. Autologous muscle stem cell injection can be an effective treatment for post-prostatectomy urinary incontinence.
AuthorsQi-sheng Yao, Zhang-qun Ye, Cong-bo Chen, Xiao-kang Wang, Wei-min Wang, Li-xin Chen
JournalZhonghua nan ke xue = National journal of andrology (Zhonghua Nan Ke Xue) Vol. 11 Issue 4 Pg. 272-4, 277 (Apr 2005) ISSN: 1009-3591 [Print] China
PMID15921257 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • beta-Galactosidase
Topics
  • Animals
  • Cell Survival
  • Cell Transplantation
  • Female
  • Muscle, Skeletal (cytology)
  • Postoperative Complications (therapy)
  • Prostatectomy
  • Rats
  • Rats, Sprague-Dawley
  • Transplantation, Autologous
  • Urethra (cytology)
  • Urinary Bladder (cytology)
  • Urinary Incontinence (etiology, therapy)
  • beta-Galactosidase (genetics)

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