Abstract | OBJECTIVE: METHODS: Skeletal muscle-derived cells (MDC) were isolated and purified by replate technique from 6 female SD rats, and then transduced with adenovirus carrying Lac-Z gene. About 5 x 10(6) of the transduced cells were injected autologously into the bladder neck of the animals. Tissues were harvested after 5 and 15 days for histological examination and X-gal staining. RESULTS: At 5 and 15 days after the autologous MDC transplantation, histological examination revealed no apparent sign of inflammation and inflammatory cell invasion, and X-gal staining showed a large number of cells dyed blue, indicating the survival of the autologous cells. CONCLUSION:
|
Authors | Qi-sheng Yao, Zhang-qun Ye, Cong-bo Chen, Xiao-kang Wang, Wei-min Wang, Li-xin Chen |
Journal | Zhonghua nan ke xue = National journal of andrology
(Zhonghua Nan Ke Xue)
Vol. 11
Issue 4
Pg. 272-4, 277
(Apr 2005)
ISSN: 1009-3591 [Print] China |
PMID | 15921257
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Animals
- Cell Survival
- Cell Transplantation
- Female
- Muscle, Skeletal
(cytology)
- Postoperative Complications
(therapy)
- Prostatectomy
- Rats
- Rats, Sprague-Dawley
- Transplantation, Autologous
- Urethra
(cytology)
- Urinary Bladder
(cytology)
- Urinary Incontinence
(etiology, therapy)
- beta-Galactosidase
(genetics)
|