Bio-artificial periosteum for severe open fracture--an experimental study of osteogenic cell/collagen sponge composite as a bio-artificial periosteum.
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| Abstract |
In an attempt to reduce complications in cases of severe open fracture, we developed a bio-artificial periosteum composed of osteogenic cells and collagen sponge. In the present study, we evaluated the osteogenic potential of the bio-artificial periosteum in vivo and in vitro. After 4-week incubation in vitro, the bio-artificial periosteum had high alkaline phosphatase activity and osteocalcin content. Moreover, energy dispersive X-ray analysis revealed numerous crystal structures consisting of P and Ca on the surface of the bio-artificial periosteum. Using a rat model for severe bone injury, we examined the bone formation process in defect sites covered with the bio-artificial periosteum. New bone formation occurred in the central part of the bone defect as well as at the bone edge. We conclude that by using the bio-artificial periosteum, the fracture site benefited from an improved osteogenic environment. These results indicate that a clinical trial to further evaluate this technique should be conducted.
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| Authors | Koji Hattori, Takafumi Yoshikawa, Yoshinori Takakura, Hideyuki Aoki, Masato Sonobe, Naohide Tomita |
| Journal | Bio-medical materials and engineering (Biomed Mater Eng) Vol. 15 Issue 3 Pg. 127-36 ( 2005) ISSN: 0959-2989 [Print] Netherlands |
| PMID | 15911994 (Publication Type: Journal Article) |
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