HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Interaction between gamma-aminobutyric acid GABAB and cannabinoid CB1 receptors in spinal pain pathways in rat.

Abstract
Antinociceptive effects of cannabinoids are mediated, in part, at the spinal level. Cannabinoid CB1 receptors are co-localized with dorsal horn interneurons containing gamma-aminobutyric acid (GABA). In this study, we investigated the interaction between intrathecally administered cannabinoid and GABA(B) receptor agonists and antagonists in the modulation of formalin-induced pain at the spinal level. Intrathecal pretreatment of rats with a cannabinoid receptor antagonist [N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1-H-pyrazole-3-carboxamide] (SR141716A, 30 microg) decreased the analgesic effect of the intrathecal administration of the GABA(B) receptor agonist, baclofen (0.125 microg and 0.25 microg). Intrathecal administration of the GABA(B) receptor antagonist, saclofen (30 microg), 10 min before administration of the cannabinoid receptor agonist (-)-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)-phenyl]-trans-4-(3-hydroxy-propyl)-cyclohexano (CP55940), did not affect the analgesia produced by the cannabinoid receptor agonist. Our results confirm that intrathecal administration of cannabinoid and GABA(B) receptor agonists have analgesic effects and that spinal antinociceptive effects of GABA(B) receptor agonists are likely through endocannabinoid modulation.
AuthorsNima Naderi, Bijan Shafaghi, Mohammad-Javad Khodayar, Mohammad-Reza Zarindast
JournalEuropean journal of pharmacology (Eur J Pharmacol) Vol. 514 Issue 2-3 Pg. 159-64 (May 09 2005) ISSN: 0014-2999 [Print] Netherlands
PMID15910802 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cyclohexanols
  • GABA Agonists
  • GABA Antagonists
  • GABA-B Receptor Agonists
  • GABA-B Receptor Antagonists
  • Piperidines
  • Pyrazoles
  • Receptor, Cannabinoid, CB1
  • Receptors, GABA-B
  • Formaldehyde
  • 3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol
  • Baclofen
  • saclofen
  • Rimonabant
Topics
  • Analysis of Variance
  • Animals
  • Baclofen (analogs & derivatives, pharmacology)
  • Cyclohexanols (pharmacology)
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Formaldehyde
  • GABA Agonists (pharmacology)
  • GABA Antagonists (pharmacology)
  • GABA-B Receptor Agonists
  • GABA-B Receptor Antagonists
  • Injections, Spinal
  • Male
  • Pain (chemically induced, physiopathology, prevention & control)
  • Piperidines (pharmacology)
  • Pyrazoles (pharmacology)
  • Rats
  • Rats, Wistar
  • Receptor, Cannabinoid, CB1 (agonists, antagonists & inhibitors, physiology)
  • Receptors, GABA-B (physiology)
  • Rimonabant
  • Signal Transduction (physiology)
  • Spinal Cord (drug effects, physiopathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: