Abstract |
In order to improve the efficacy of current vaccine candidates against HIV/ AIDS, we sought to strengthen the induction of immune responses via simultaneous in vivo mobilization of dendritic cells using a chimeric Flt-3 and G-CSF receptor agonists (ProGP). We investigated ProGP treatment in combination with two DNA immunizations encoding HIV-Env89.6, SIV-Gag proteins to increase the priming of immune responses. Administration of this Flt-3/ G-CSF chimera elicited marked increases in numbers of both plasmacytoid and myeloid dendritic cells. However, there was no increase seen in T-cell responses either directly following the DNA immunization or after further boosting with MVA vectors expressing HIV-Env89.6p, SIV-Gag. After challenge with SHIV89.6p all animals became infected and no differences were seen between the ProGP treated versus the control group with regard to plasma virus load or CD4 T-cell count. We conclude that besides mobilization of dendritic cells additional stimuli to induce dendritic cell maturation may be needed for avid boosting of antigen specific immune activation.
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Authors | Gerrit Koopman, D Mortier, H Niphuis, Ann M Farese, Larry E Kahn, Dean Mann, Ralf Wagner, Thomas J MacVittie, Susan L Woulfe, Jonathan L Heeney |
Journal | Vaccine
(Vaccine)
Vol. 23
Issue 33
Pg. 4195-202
(Jul 21 2005)
ISSN: 0264-410X [Print] Netherlands |
PMID | 15896883
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AIDS Vaccines
- Antibodies, Viral
- HIV Antigens
- Proto-Oncogene Proteins
- Receptors, Granulocyte Colony-Stimulating Factor
- Recombinant Fusion Proteins
- FLT3 protein, human
- Receptor Protein-Tyrosine Kinases
- fms-Like Tyrosine Kinase 3
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Topics |
- AIDS Vaccines
(administration & dosage, genetics, immunology)
- Animals
- Antibodies, Viral
(blood)
- Antigen-Presenting Cells
- Dendritic Cells
(drug effects, immunology)
- HIV Antigens
(administration & dosage)
- HIV Infections
(immunology, prevention & control)
- HIV-1
(chemistry, immunology)
- Humans
- Immunity
(drug effects)
- Immunization
- Macaca mulatta
- Proto-Oncogene Proteins
(agonists, genetics, immunology)
- Receptor Protein-Tyrosine Kinases
(genetics, immunology)
- Receptors, Granulocyte Colony-Stimulating Factor
(agonists)
- Recombinant Fusion Proteins
(immunology)
- fms-Like Tyrosine Kinase 3
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