Abstract | BACKGROUND: AIMS: We assessed whether inactivation of NFkappaB in the liver could attenuate total hepatic warm ischaemia/ reperfusion injury. METHODS: We studied rats with hepatic overexpression of inhibitor kappaBalpha super-repressor ( IkappaBalpha SR) caused by a transgene introduced using an adenoviral vector. Hepatic ischaemia/ reperfusion injury was induced under warm conditions by total occlusion of hepatoduodenal ligament structures for 20 minutes, followed by reperfusion. Controls included uninfected and control virus (AdLacZ) infected rats. RESULTS: CONCLUSIONS: Adenoviral transfer of the IkappaBalpha SR gene in the liver ameliorates short term warm ischaemia/ reperfusion injury, possibly through attenuation of hepatic macrophage activation.
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Authors | H Suetsugu, Y Iimuro, T Uehara, T Nishio, N Harada, M Yoshida, E Hatano, G Son, J Fujimoto, Y Yamaoka |
Journal | Gut
(Gut)
Vol. 54
Issue 6
Pg. 835-42
(Jun 2005)
ISSN: 0017-5749 [Print] England |
PMID | 15888794
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aldehydes
- I-kappa B Proteins
- NF-kappa B
- Nfkbia protein, rat
- Tumor Necrosis Factor-alpha
- NF-KappaB Inhibitor alpha
- 3-nitrotyrosine
- Tyrosine
- 4-hydroxy-2-nonenal
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Topics |
- Adenoviridae
- Aldehydes
(metabolism)
- Animals
- Blotting, Western
- Gene Transfer Techniques
- Hepatocytes
(metabolism)
- I-kappa B Proteins
(pharmacology)
- Kupffer Cells
(metabolism)
- Liver
(blood supply, metabolism, pathology)
- Male
- NF-KappaB Inhibitor alpha
- NF-kappa B
(antagonists & inhibitors)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(pathology, prevention & control)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Transfection
- Tumor Necrosis Factor-alpha
- Tyrosine
(analogs & derivatives, metabolism)
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