Antigenotoxic potential of glucomannan on four model test systems.

Abstract

Antimutagenic, anticlastogenic, and bioprotective effect of polysaccharide glucomannan (GM) isolated from Candida utilis was evaluated in four model test systems. The antimutagenic effect of GM against 9-aminoacridine (9-AA)- and sodium azide (NaN3)-induced mutagenicity was revealed in the Salmonella typhimurium strains TA97 and TA100, respectively. GM showed anticlastogenic effect against N-nitroso-N'-methylurea (NMU) induced chromosome aberrations in the Vicia sativa assay. The bioprotective effect of GM co-treated with methyl-methane-sulphonate (MMS) was also established in Chlamydomonas reinhardtii repair deficient strains uvs10 and uvs14. The statistically significant antimutagenic potential of GM was not proved against 4-nitro-quinoline-1-oxide (4-NQO)-induced mutagenicity in Saccharomyces cerevisiae D7 assay. It may be due to bioprotectivity of alpha-mannan and beta-glucan, which are integral part of S. cerevisiae cell walls. Due to the good water solubility, low molecular weight (30 kDa), antimutagenic/anticlastogenic, and bioprotective activity against chemical compounds differing in mode of action, GM appears to be a promising natural protective (antimutagenic) agent.

• Authors: V Vlcková, V Dúhová, S Svidová, A Farkassová, S Kamasová, D Vlcek, G Kogan, P Rauko, E Miadoková
• Journal: Cell biology and toxicology (Cell Biol Toxicol) Vol. 20 Issue 6 Pg. 325-32 (Nov 2004) ISSN: 0742-2091 [Print] Netherlands
• PMID: 15868477 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimutagenic Agents
• Mannans
• Mutagens
• (1-6)-alpha-glucomannan
• 4-Nitroquinoline-1-oxide
• Methylnitrosourea
• Aminacrine
• Sodium Azide
• Methyl Methanesulfonate

Topics

• 4-Nitroquinoline-1-oxide (pharmacology)
• Aminacrine (pharmacology)
• Animals
• Antimutagenic Agents (pharmacology)
• Candida (chemistry)
• Cell Division (drug effects, genetics)
• Chlamydomonas reinhardtii (cytology, drug effects, genetics)
• Chromosome Aberrations (drug effects)
• Crossing Over, Genetic (drug effects)
• DNA Damage (drug effects)
• Mannans (pharmacology)
• Methyl Methanesulfonate (pharmacology)
• Methylnitrosourea (pharmacology)
• Mutagenicity Tests (methods)
• Mutagens (pharmacology)
• Saccharomyces cerevisiae (drug effects, genetics)
• Salmonella typhimurium (drug effects, genetics)
• Sodium Azide (pharmacology)
• Vicia sativa (cytology, drug effects, genetics)