Inhibitory effects of herbal drugs on the growth of human ovarian cancer cell lines through the induction of apoptosis.

Abstract

OBJECTIVE:

In order to develop and search for more effective and safe treatments for early and advanced stages of ovarian cancer, we examined the direct effects of four extracts of Chinese herbal drugs on ovarian cancer cells in vitro.

METHODS:

The growth inhibition of four herbal drugs on a total of six cell lines of human ovarian cancer cells was determined by a Cell Counting Kit-8 by counting viable cells. Apoptotic cells induced by herbal drugs were detected by using MEBCYTO Apoptosis Kit. All experiments were performed in triplicate. The significance of the difference was analyzed with a two-sided Student's t test. A P value less than 0.05 was accepted as statistically significant.

RESULTS:

The MN, A2780, and KF cell lines exhibited significant growth inhibition in the presence of Sho-saiko-to concentrations of 150 microg/ml, 300 microg/ml, and 500 microg/ml, respectively, and at the concentration of 1000 microg/ml, Sho-saiko-to demonstrated a significant apoptotic induction effect on all six kinds of ovarian cancer cell lines. This concentration is the same as the blood concentration attained when 7.5 g of Sho-saiko-to per day is orally administered and all absorbed.

CONCLUSIONS:

Sho-saiko-to exhibited significant growth inhibition of ovarian cancer cell lines, and the mechanisms of the inhibitory effects can be attributed, in part, to apoptosis induced by Sho-saiko-to.

• Authors: Kun Zhu, Ichio Fukasawa, Makiko Furuno, Fujiyuki Inaba, Tatsuo Yamazaki, Tetsu Kamemori, Nobuaki Kousaka, Yoriko Ota, Masatoshi Hayashi, Toshiyuki Maehama, Noriyuki Inaba
• Journal: Gynecologic oncology (Gynecol Oncol) Vol. 97 Issue 2 Pg. 405-9 (May 2005) ISSN: 0090-8258 [Print] United States
• PMID: 15863137 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• bu-zhong-yi-qi-tang
• shosaiko-to

Topics

• Apoptosis (drug effects)
• Cell Growth Processes (drug effects)
• Cell Line, Tumor
• Drugs, Chinese Herbal (pharmacology)
• Female
• Humans
• Ovarian Neoplasms (drug therapy, pathology)
• Phytotherapy (methods)