D-002 is a mixture of higher aliphatic primary
alcohols isolated from
beeswax, wherein
triacontanol is the most abundant alcohol, with
antioxidant and anti-
ulcer properties. Since compounds with cytoprotective and
antioxidant effects can improve healing of
gastroduodenal ulcer induced by noxious agents, this work investigated the healing effect of
D- 002 on acute and chronic
gastric ulcers induced with
indomethacin and
acetic acid, respectively, in rats. Acute
gastric ulcer was induced with single oral doses of
indomethacin (20 mg/kg). Treatments with
D-002 at 50, 100, and 200 mg/kg or vehicle were administered 3 hours after
ulcer induction. Three hours later, rats were sacrificed, and the stomach was removed for quantifying the lesions. Chronic
gastric ulcer was induced by 50 microL of 80%
acetic acid application on the anterior serosal surface of the glandular stomach during 20 seconds. Twenty-four hours later
D-002 at 50, 100, and 200 mg/kg or vehicle was administered for 5 days. At the end of the treatment, animals were fasted for 24 hours and sacrificed, the stomachs were removed, and the lesions were quantified.
D-002 orally administered at 100 and 200 mg/kg acutely significantly healed
gastric ulcers induced with
indomethacin by 39% and 56% compared with positive controls, respectively. Also,
D-002 at 200 mg/kg, but not at 50 or 100 mg/kg, administered orally for 5 days after
ulcer induction exerted a significant healing effect (65.8% inhibition) in
gastric ulcers induced with
acetic acid. In conclusion, this work demonstrated that
D-002 administered after
ulcer induction induced effective healing of acute and chronic
gastric ulcers provoked by, respectively,
indomethacin and
acetic acid.