Abstract | CONTEXT: OBJECTIVE: DESIGN: Left ventricular mass (LVM) was calculated through electrocardiogram reading in normotensive grade III obese patients (World Health Organization classification) undergoing bariatric surgery [laparoscopic adjustable gastric banding (LAGB)] at baseline and 1 yr later. The control group was composed of healthy lean normotensive subjects. Leptin receptors were detected by PCR and immunocytochemistry in human heart biopsies. SETTING: This study was performed at university hospitals. PATIENTS: Thirty-one grade 3 obese patients and 30 healthy nonobese normotensive, age- and sex-matched control subjects were studied. INTERVENTION: Obese subjects underwent LAGB to induce weight loss and were evaluated at baseline and after 1 yr. RESULTS: LVM, plasma leptin, glucose, insulin levels, and homeostasis model assessment index were higher in obese than in lean controls (P < 0.01); at univariate regression analysis, LVM correlated with body mass index, leptin, and homeostasis model assessment index; at multiple regression analysis, LVM only correlated with leptin levels (P = 0.001). Obese subjects were reevaluated 1 yr after LAGB, when their body mass index changed from 46.2 +/- 1.24 to 36.6 +/- 1.05 kg/m(2) (P < 0.01); the decrease in LVM correlated only with the decrease in leptin levels (P < 0.01). We demonstrated that long and short isoforms of the leptin receptor and intracellular proteins mediating leptin signaling were expressed in human heart by RT-PCR, immunocytochemistry, or both methods. CONCLUSIONS:
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Authors | Lucia Perego, Pierluigi Pizzocri, Domenico Corradi, Francesco Maisano, Michele Paganelli, Paolo Fiorina, Michelangela Barbieri, Alberto Morabito, Giuseppe Paolisso, Franco Folli, Antonio E Pontiroli |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 90
Issue 7
Pg. 4087-93
(Jul 2005)
ISSN: 0021-972X [Print] United States |
PMID | 15855267
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- LEPR protein, human
- Leptin
- Receptors, Cell Surface
- Receptors, Leptin
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Topics |
- Adult
- Body Mass Index
- Female
- Humans
- Hypertrophy, Left Ventricular
(etiology)
- Leptin
(blood, physiology)
- Male
- Obesity, Morbid
(blood, surgery)
- Receptors, Cell Surface
(analysis)
- Receptors, Leptin
- Regression Analysis
- Weight Loss
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