Induction of humoral responses specific for paraneoplastic cerebellar degeneration-associated antigen by whole recombinant yeast immunization.

Abstract

Paraneoplastic cerebellar degeneration (PCD) is a potent autoimmune disorder in which antigen-driven responses toward the onconeural antigen are assumed to occur in patients. Yeast cell wall has adjuvant capacity and provides immunostimulatory effects of the antigen expressing in viable cells. The recombinant yeast expressing the PCD-associated antigen may become an immunogen for inducing PCD-associated autoimmunity in mice. We attempted to induce autoimmune responses with whole recombinant yeast expressing PCD-associated antigen. SJL/J strain of mouse is found to be a responder to the major epitope on the antigen for anti-Purkinje cell antibodies, and whole recombinant yeast could induce cellular and humoral autoimmune responses in vivo ion SJL/J mice. The immunization technique based on the recombinant yeast expressing a PCD-associated antigen provides a new tool for analyzing the underlying immunological pathomechanisms of PCD.

• Authors: Misuzu Saiki, Koichiro Sakai, Shinji Saiki, Yoko Kitagawa, Megumi Nakanishi, Genjiro Hirose
• Journal: Journal of autoimmunity (J Autoimmun) Vol. 24 Issue 3 Pg. 203-8 (May 2005) ISSN: 0896-8411 [Print] England
• PMID: 15848042 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• Autoantigens
• Nerve Tissue Proteins
• Pcd17 protein, mouse

Topics

• Animals
• Antigens, Surface (immunology)
• Autoantigens (immunology)
• Autoimmune Diseases (etiology, immunology)
• Disease Models, Animal
• Immunization
• Mice
• Nerve Tissue Proteins (administration & dosage, immunology)
• Paraneoplastic Cerebellar Degeneration (immunology, pathology)
• Purkinje Cells (immunology, pathology)
• Saccharomyces cerevisiae (immunology)