Abstract | BACKGROUND & AIMS:
Basic fibroblast growth factor (bFGF) is a promising therapeutic agent for various diseases. It remains unclear, however, whether bFGF is effective for the treatment of inflammatory bowel disease. The aim of this study was to examine the efficacy of bFGF on 2 experimental murine colitis models and to investigate its molecular mechanisms. METHODS: RESULTS:
Rectal administration of hrbFGF ameliorated DSS-induced colitis in a dose-dependent manner. Gene expression of TNF-alpha was significantly reduced in the colonic tissues of mice with DSS-induced colitis treated with hrbFGF, whereas MUC2 and ITF messenger RNA expression was up-regulated. Rectal administration of hrbFGF significantly improved the survival rate of mice with TNBS-induced colitis and partially ameliorated colitis. hrbFGF significantly increased the number of Ki-67-positive cells in the colonic epithelium of normal mice, and up-regulated the gene expression of COX-2, TGF-beta, MUC2, ITF, and VEGF in the colonic tissues. CONCLUSIONS:
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Authors | Minoru Matsuura, Kazuichi Okazaki, Akiyoshi Nishio, Hiroshi Nakase, Hiroyuki Tamaki, Kazushige Uchida, Toshiki Nishi, Masanori Asada, Kimio Kawasaki, Toshiro Fukui, Hazuki Yoshizawa, Shinya Ohashi, Satoko Inoue, Chiharu Kawanami, Hiroshi Hiai, Yasuhiko Tabata, Tsutomu Chiba |
Journal | Gastroenterology
(Gastroenterology)
Vol. 128
Issue 4
Pg. 975-86
(Apr 2005)
ISSN: 0016-5085 [Print] United States |
PMID | 15825079
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Inflammation Mediators
- Ki-67 Antigen
- Recombinant Proteins
- Fibroblast Growth Factor 2
- Trinitrobenzenesulfonic Acid
- Dextran Sulfate
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Topics |
- Administration, Rectal
- Animals
- Body Weight
(drug effects)
- Colitis
(chemically induced, metabolism, pathology)
- Colon
(pathology)
- Cytokines
(metabolism)
- Dextran Sulfate
- Female
- Fibroblast Growth Factor 2
(administration & dosage, pharmacology)
- Gene Expression
- Humans
- Inflammation Mediators
(metabolism)
- Intestinal Mucosa
(drug effects, pathology)
- Ki-67 Antigen
(metabolism)
- Mice
- Mice, Inbred C57BL
- Organ Size
(drug effects)
- Recombinant Proteins
(administration & dosage, pharmacology)
- Survival Analysis
- Trinitrobenzenesulfonic Acid
- Wound Healing
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