Abstract | AIM: METHODS: RESULTS: Both fibril type I and IV collagen significantly increased type I collagenase activity about two-fold compared with no fibril collagen. The effects of the fibril collagen were not affected by the coating condition. There was no significant difference in the effects on collagenase activity between cells cultured in medium containing fibril type I collagen and those cultured in the presence of type IV collagen. Both types of fibril collagen significantly increased MMP-1 production, and showed more than 10-fold higher levels of MMP-1 than the control. The enhanced MMP-1 production by fibril collagens was unaffected by the coating condition. By contrast, TIMP-1 production was not changed by the addition of fibril type I or IV collagen, and neither was it affected by the coating conditions. Coating with type I collagen significantly suppressed MMP-1 production by almost one-tenth compared with no coating. By contrast, TIMP-1 production was not affected by either the absence of a collagen coat or by increasing the concentration of the coating collagen. CONCLUSION: These results indicated that, in HLE cells, fibril- and fixed- collagen have opposite effects on MMP-1 production without affecting TIMP production. Fibril collagen induced collagenase activity by up-regulation of MMP-1 production without affecting TIMP-1 production. By contrast, fixed collagen reduced MMP-1 production. Our results suggest that hepatocytes might also play an important role in the regulation of the hepatic fibrosis alongside HSC.
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Authors | Makoto Nakamuta, Kazuhiro Kotoh, Munechika Enjoji, Hajime Nawata |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 11
Issue 15
Pg. 2264-8
(Apr 21 2005)
ISSN: 1007-9327 [Print] United States |
PMID | 15818737
(Publication Type: Journal Article)
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Chemical References |
- Collagen Type I
- Collagen Type IV
- Fibril-Associated Collagens
- Tissue Inhibitor of Metalloproteinase-1
- Matrix Metalloproteinase 1
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Topics |
- Carcinoma, Hepatocellular
(metabolism, pathology)
- Cell Line, Tumor
- Collagen Type I
(pharmacology)
- Collagen Type IV
(pharmacology)
- Fibril-Associated Collagens
(pharmacology)
- Hepatocytes
(cytology, drug effects, metabolism)
- Humans
- Liver Cirrhosis
(metabolism)
- Liver Neoplasms
(metabolism, pathology)
- Matrix Metalloproteinase 1
(metabolism)
- Tissue Inhibitor of Metalloproteinase-1
(metabolism)
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