The relationship between bone metastasis from human breast cancer and integrin alpha(v)beta3 expression.

Abstract

BACKGROUND:

Osteoclast activation plays an essential role in the development of bone metastasis (BM). Integrin alpha(v)beta3 mediates the attachment of osteoclasts to bone matrix, and it is overexpressed in bone-residing breast cancer cells. We studied BM from breast cancer in relation to its Integrin alpha(v)beta3 expression.

MATERIALS AND METHODS:

Integrin alpha(v)beta3 expression in 4 human breast cancer cell lines (MDA-MB-231, MDA-MB-435, MKL-4 and T-47D) was determined by immunohistochemistry, flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR).

RESULTS:

Integrin alpha(v)beta3 was expressed in MDA-MB-231 and MDA-MB-435, but not in MKL-4 or T-47D, using both immunohistochemistry and flow cytometry. By RT-PCR, positive bands for both alpha(v) and beta3 subunits were identified in MDA-MB-231 and 435, while only the alpha(v) subunit mRNA was present in MKL-4 and T-47D. BM was common in vivo following inoculation with MDA-MB-231 or 435, but not with MKL-4.

CONCLUSION:

Integrin alpha(v)beta3 expression appears to play a key role in the development of BM from breast cancer.

• Authors: Shin Takayama, Seiichiro Ishii, Tadashi Ikeda, Shigeru Masamura, Masakazu Doi, Masaki Kitajima
• Journal: Anticancer research (Anticancer Res) 2005 Jan-Feb Vol. 25 Issue 1A Pg. 79-83 ISSN: 0250-7005 [Print] Greece
• PMID: 15816522 (Publication Type: Journal Article)

Chemical References

• Integrin alphaVbeta3

Topics

• Animals
• Bone Neoplasms (metabolism, secondary)
• Breast Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Humans
• Immunohistochemistry
• Integrin alphaVbeta3 (biosynthesis)
• Neoplasm Transplantation
• Rats
• Rats, Nude
• Transplantation, Heterologous