Abstract |
Combined immunization with gene-based and protein-based vaccines can increase vaccine effectiveness. We previously demonstrated, using a murine model for Japanese encephalitis (JE), that simultaneous immunization with a DNA vaccine (pcJEME) by the intramuscular route and a protein vaccine consisting of subviral extracellular particles (EPs) by the subcutaneous route provided a synergistic increase in immunogenicities of these vaccines. Here, we investigated a novel immunization protocol consisting of a single inoculation with a mixture of DNA and protein vaccines using a needle-free jet injector. Immunization of ddY mice with 1 microg of pcJEME mixed with 1 microg of EPs or a 1/100 dose of commercial inactivated JE vaccine (JEVAX) induced neutralizing antibody titers of 1:40 to 1:80 (90% plaque reduction) 6 weeks after immunization, whereas immunization with DNA or protein alone only induced low titers (< or =1:10). Co-immunization with pcDNA3, a CpGcontaining vector of the vaccine plasmid, increased immunogenicity of JEVAX to some extent. IgG1/ IgG2a isotype profiles supported increased production of EPs in pcJEME-inoculated mice by needle-free injection and an adjuvant effect of the vector on immunogenicity of JEVAX.
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Authors | Jun-Ichi Imoto, Eiji Konishi |
Journal | Viral immunology
(Viral Immunol)
Vol. 18
Issue 1
Pg. 205-12
( 2005)
ISSN: 0882-8245 [Print] United States |
PMID | 15802965
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Viral
- Drug Combinations
- Vaccines, DNA
- Vaccines, Synthetic
- Viral Vaccines
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Topics |
- Adjuvants, Immunologic
- Animals
- Antibodies, Viral
(blood)
- CpG Islands
(immunology)
- Disease Models, Animal
- Drug Combinations
- Encephalitis Virus, Japanese
(genetics)
- Encephalitis, Japanese
(blood, immunology, prevention & control)
- Injections, Jet
- Mice
- Neutralization Tests
- Vaccination
- Vaccines, DNA
(administration & dosage)
- Vaccines, Synthetic
(administration & dosage)
- Viral Vaccines
(administration & dosage)
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