Inhibitory effects of Stephania tetrandra S. Moore on free radical-induced lysis of rat red blood cells.

Abstract	Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 microg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.
Authors	Nobuyasu Sekiya, Hiroaki Hikiami, Koichi Yokoyama, Kazufumi Kouta, Iwao Sakakibara, Yutaka Shimada, Katsutoshi Terasawa
Journal	Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 28 Issue 4 Pg. 667-70 (Apr 2005) ISSN: 0918-6158 [Print] Japan
PMID	15802807 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Alkaloids Antioxidants Benzylisoquinolines Free Radical Scavengers Free Radicals Plant Extracts Berberine cyclanoline tetrandrine fangchinoline
Topics	Alkaloids (pharmacology) Animals Antioxidants (pharmacology) Benzylisoquinolines (pharmacology) Berberine (analogs & derivatives, pharmacology) Dose-Response Relationship, Drug Free Radical Scavengers (pharmacology) Free Radicals (metabolism) Hemolysis (drug effects) In Vitro Techniques Male Molecular Structure Plant Extracts (pharmacology) Rats Rats, Wistar Stephania tetrandra (chemistry) Time Factors

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